|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TXN2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12557-ACG|
|Human TXN2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12557-ACR|
|Human TXN2 ORF mammalian expression plasmid, N-GFPSpark tag||HG12557-ANG|
|Human TXN2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12557-ANR|
|Human TXN2 ORF mammalian expression plasmid, C-Flag tag||HG12557-CF|
|Human TXN2 ORF mammalian expression plasmid, C-His tag||HG12557-CH|
|Human TXN2 ORF mammalian expression plasmid, C-Myc tag||HG12557-CM|
|Human TXN2 ORF mammalian expression plasmid, C-HA tag||HG12557-CY|
|Human TXN2 Gene cDNA clone plasmid||HG12557-G|
|Human TXN2 ORF mammalian expression plasmid, N-Flag tag||HG12557-NF|
|Human TXN2 ORF mammalian expression plasmid, N-His tag||HG12557-NH|
|Human TXN2 ORF mammalian expression plasmid, N-Myc tag||HG12557-NM|
|Human TXN2 ORF mammalian expression plasmid, N-HA tag||HG12557-NY|
|Human TXN2 natural ORF mammalian expression plasmid||HG12557-UT|
|Learn more about expression Vectors|
Thioredoxin-2, also known as TXN2, MTRX and TRX2, is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which, as trypanothione:peroxiredoxin oxidoreductases, constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2 / TXN2 contains one thioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2 / TXN2 is a small redox protein important in cellular antioxidant defenses, as well as in the regulation of apoptosis. Thioredoxin-2 / TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2 / TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2 / TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2 / TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.