|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human IFNA1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12341-ACG|
|Human IFNA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12341-ACR|
|Human IFNA1 ORF mammalian expression plasmid, C-Flag tag||HG12341-CF|
|Human IFNA1 ORF mammalian expression plasmid, C-His tag||HG12341-CH|
|Human IFNA1 ORF mammalian expression plasmid, C-Myc tag||HG12341-CM|
|Human IFNA1 ORF mammalian expression plasmid, C-HA tag||HG12341-CY|
|Human IFNA1 Gene cDNA clone plasmid||HG12341-G|
|Human IFNA1 natural ORF mammalian expression plasmid||HG12341-G-N|
|Human IFNA1 ORF mammalian expression plasmid, N-Flag tag||HG12341-NF|
|Human IFNA1 ORF mammalian expression plasmid, N-His tag||HG12341-NH|
|Human IFNA1 ORF mammalian expression plasmid, N-Myc tag||HG12341-NM|
|Human IFNA1 ORF mammalian expression plasmid, N-HA tag||HG12341-NY|
|Human IFNA1 natural ORF mammalian expression plasmid||HG12341-UT|
|Learn more about expression Vectors|
IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells. They belong to the large class of glycoproteins known as cytokines. IFNs stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they also increase the ability of uninfected host cells to resist new infection by virus.Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has antiviral activities.