|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CD47 ORF mammalian expression plasmid, C-GFPSpark tag||HG12283-ACG|
|Human CD47 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12283-ACR|
|Human CD47 ORF mammalian expression plasmid, C-Flag tag||HG12283-CF|
|Human CD47 ORF mammalian expression plasmid, C-His tag||HG12283-CH|
|Human CD47 ORF mammalian expression plasmid, C-Myc tag||HG12283-CM|
|Human CD47 ORF mammalian expression plasmid, C-HA tag||HG12283-CY|
|Human CD47 Gene cDNA clone plasmid||HG12283-G|
|Human CD47 natural ORF mammalian expression plasmid||HG12283-G-N|
|Human CD47 ORF mammalian expression plasmid, N-Flag tag||HG12283-NF|
|Human CD47 ORF mammalian expression plasmid, N-His tag||HG12283-NH|
|Human CD47 ORF mammalian expression plasmid, N-Myc tag||HG12283-NM|
|Human CD47 ORF mammalian expression plasmid, N-HA tag||HG12283-NY|
|Human CD47 natural ORF mammalian expression plasmid||HG12283-UT|
|Learn more about expression Vectors|
CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.