|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CD33 ORF mammalian expression plasmid, C-GFPSpark tag||HG12238-ACG|
|Human CD33 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12238-ACR|
|Human CD33 ORF mammalian expression plasmid, C-Flag tag||HG12238-CF|
|Human CD33 ORF mammalian expression plasmid, C-His tag||HG12238-CH|
|Human CD33 ORF mammalian expression plasmid, C-Myc tag||HG12238-CM|
|Human CD33 ORF mammalian expression plasmid, C-HA tag||HG12238-CY|
|Human CD33 Gene cDNA clone plasmid||HG12238-G|
|Human CD33 ORF mammalian expression plasmid, N-Flag tag||HG12238-NF|
|Human CD33 ORF mammalian expression plasmid, N-His tag||HG12238-NH|
|Human CD33 ORF mammalian expression plasmid, N-Myc tag||HG12238-NM|
|Human CD33 ORF mammalian expression plasmid, N-HA tag||HG12238-NY|
|Human CD33 natural ORF mammalian expression plasmid||HG12238-UT|
|Learn more about expression Vectors|
Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.