|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human BTC ORF mammalian expression plasmid, C-GFPSpark tag||HG12192-ACG|
|Human BTC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12192-ACR|
|Human BTC ORF mammalian expression plasmid, C-Flag tag||HG12192-CF|
|Human BTC ORF mammalian expression plasmid, C-His tag||HG12192-CH|
|Human BTC ORF mammalian expression plasmid, C-Myc tag||HG12192-CM|
|Human BTC ORF mammalian expression plasmid, C-HA tag||HG12192-CY|
|Human BTC Gene cDNA clone plasmid||HG12192-G|
|Human BTC ORF mammalian expression plasmid, Flag tag||HG12192-G-F|
|Human BTC ORF mammalian expression plasmid, N-Flag tag||HG12192-NF|
|Human BTC ORF mammalian expression plasmid, N-His tag||HG12192-NH|
|Human BTC ORF mammalian expression plasmid, N-Myc tag||HG12192-NM|
|Human BTC ORF mammalian expression plasmid, N-HA tag||HG12192-NY|
|Human BTC natural ORF mammalian expression plasmid||HG12192-UT|
|Learn more about expression Vectors|
Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.