|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human INHBB ORF mammalian expression plasmid, C-GFPSpark tag||HG10814-ACG|
|Human INHBB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10814-ACR|
|Human INHBB ORF mammalian expression plasmid, C-Flag tag||HG10814-CF|
|Human INHBB ORF mammalian expression plasmid, C-His tag||HG10814-CH|
|Human INHBB ORF mammalian expression plasmid, C-Myc tag||HG10814-CM|
|Human INHBB ORF mammalian expression plasmid, C-HA tag||HG10814-CY|
|Human INHBB Gene cDNA clone plasmid||HG10814-M|
|Human INHBB ORF mammalian expression plasmid, N-Flag tag||HG10814-NF|
|Human INHBB ORF mammalian expression plasmid, N-His tag||HG10814-NH|
|Human INHBB ORF mammalian expression plasmid, N-Myc tag||HG10814-NM|
|Human INHBB ORF mammalian expression plasmid, N-HA tag||HG10814-NY|
|Human INHBB natural ORF mammalian expression plasmid||HG10814-UT|
|Learn more about expression Vectors|
Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. The activin and inhibin protein complexes are both dimeric in structure, and, in each complex, the two monomers are linked to one another by a single disulfide bond. Activin is composed of two ß subunits, ßA ßA (activin A), ßB ßB (activin B), or ßA ßB (activin AB). Inhibin is composed of an alpha and one of two ß subunits, ßA (inhibin A) or ßB (inhibin B). Activins are produced in many cell types and organs, such as gonads, pituitary gland, and placenta. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. In addition, Activin plays a role in wound repair and skin morphogenesis. Activin is strongly expressed in wounded skin, and overexpression of activin in epidermis of transgenic mice improves wound healing and enhances scar formation. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and kidney. There is also evidence showed that lack of activin during development results in neural developmental defects.