|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human BRK ORF mammalian expression plasmid, C-GFPSpark tag||HG10682-ACG|
|Human BRK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10682-ACR|
|Human BRK ORF mammalian expression plasmid, N-GFPSpark tag||HG10682-ANG|
|Human BRK ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10682-ANR|
|Human BRK ORF mammalian expression plasmid, C-Flag tag||HG10682-CF|
|Human BRK ORF mammalian expression plasmid, C-His tag||HG10682-CH|
|Human BRK ORF mammalian expression plasmid, C-Myc tag||HG10682-CM|
|Human BRK ORF mammalian expression plasmid, C-HA tag||HG10682-CY|
|Human BRK Gene cDNA clone plasmid||HG10682-M|
|Human BRK ORF mammalian expression plasmid, N-Flag tag||HG10682-NF|
|Human BRK ORF mammalian expression plasmid, N-His tag||HG10682-NH|
|Human BRK ORF mammalian expression plasmid, N-Myc tag||HG10682-NM|
|Human BRK ORF mammalian expression plasmid, N-HA tag||HG10682-NY|
|Human BRK natural ORF mammalian expression plasmid||HG10682-UT|
|Learn more about expression Vectors|
Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.