|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human IL17A ORF mammalian expression plasmid, C-GFPSpark tag||HG12047-ACG|
|Human IL17A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12047-ACR|
|Human IL17A ORF mammalian expression plasmid, C-Flag tag||HG12047-CF|
|Human IL17A ORF mammalian expression plasmid, C-His tag||HG12047-CH|
|Human IL17A ORF mammalian expression plasmid, C-Myc tag||HG12047-CM|
|Human IL17A ORF mammalian expression plasmid, C-HA tag||HG12047-CY|
|Human IL17A Gene cDNA clone plasmid||HG12047-G|
|Human IL17A ORF mammalian expression plasmid, N-Flag tag||HG12047-NF|
|Human IL17A ORF mammalian expression plasmid, N-His tag||HG12047-NH|
|Human IL17A ORF mammalian expression plasmid, N-Myc tag||HG12047-NM|
|Human IL17A ORF mammalian expression plasmid, N-HA tag||HG12047-NY|
|Human IL17A natural ORF mammalian expression plasmid||HG12047-UT|
|Learn more about expression Vectors|
IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.