|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human NQO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12046-ACG|
|Human NQO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12046-ACR|
|Human NQO1 ORF mammalian expression plasmid, N-GFPSpark tag||HG12046-ANG|
|Human NQO1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12046-ANR|
|Human NQO1 ORF mammalian expression plasmid, C-Flag tag||HG12046-CF|
|Human NQO1 ORF mammalian expression plasmid, C-His tag||HG12046-CH|
|Human NQO1 ORF mammalian expression plasmid, C-Myc tag||HG12046-CM|
|Human NQO1 ORF mammalian expression plasmid, C-HA tag||HG12046-CY|
|Human NQO1 Gene cDNA clone plasmid||HG12046-G|
|Human NQO1 ORF mammalian expression plasmid, N-Flag tag||HG12046-NF|
|Human NQO1 ORF mammalian expression plasmid, N-His tag||HG12046-NH|
|Human NQO1 ORF mammalian expression plasmid, N-Myc tag||HG12046-NM|
|Human NQO1 ORF mammalian expression plasmid, N-HA tag||HG12046-NY|
|Human NQO1 natural ORF mammalian expression plasmid||HG12046-UT|
|Learn more about expression Vectors|
NQO1 gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. NQO1 forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in NQO1 gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recent pharmacological research suggests feasibility of genotype-directed redox chemotherapeutic intervention targeting NQO1 breast cancer, a common missense genotype encoding a functionally impaired NQO1 protein.