|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human VDR ORF mammalian expression plasmid, C-GFPSpark tag||HG12025-ACG|
|Human VDR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12025-ACR|
|Human VDR ORF mammalian expression plasmid, N-GFPSpark tag||HG12025-ANG|
|Human VDR ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12025-ANR|
|Human VDR ORF mammalian expression plasmid, C-Flag tag||HG12025-CF|
|Human VDR ORF mammalian expression plasmid, C-His tag||HG12025-CH|
|Human VDR ORF mammalian expression plasmid, C-Myc tag||HG12025-CM|
|Human VDR ORF mammalian expression plasmid, C-HA tag||HG12025-CY|
|Human VDR Gene cDNA clone plasmid||HG12025-G|
|Human VDR natural ORF mammalian expression plasmid||HG12025-G-N|
|Human VDR ORF mammalian expression plasmid, N-Flag tag||HG12025-NF|
|Human VDR ORF mammalian expression plasmid, N-His tag||HG12025-NH|
|Human VDR ORF mammalian expression plasmid, N-Myc tag||HG12025-NM|
|Human VDR ORF mammalian expression plasmid, N-HA tag||HG12025-NY|
|Human VDR natural ORF mammalian expression plasmid||HG12025-UT|
|Learn more about expression Vectors|
VDR (vitamin D(1,25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1,25(OH)2D3) and induce expression of the 1,25(OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.