|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human GREM1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10632-ACG|
|Human GREM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10632-ACR|
|Human GREM1 ORF mammalian expression plasmid, C-Flag tag||HG10632-CF|
|Human GREM1 ORF mammalian expression plasmid, C-His tag||HG10632-CH|
|Human GREM1 ORF mammalian expression plasmid, C-Myc tag||HG10632-CM|
|Human GREM1 ORF mammalian expression plasmid, C-HA tag||HG10632-CY|
|Human GREM1 Gene cDNA clone plasmid||HG10632-G|
|Human GREM1 ORF mammalian expression plasmid, N-Flag tag||HG10632-NF|
|Human GREM1 ORF mammalian expression plasmid, N-His tag||HG10632-NH|
|Human GREM1 ORF mammalian expression plasmid, N-Myc tag||HG10632-NM|
|Human GREM1 ORF mammalian expression plasmid, N-HA tag||HG10632-NY|
|Human GREM1 natural ORF mammalian expression plasmid||HG10632-UT|
|Learn more about expression Vectors|
GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. GREM1 is highly expressed in small intestine, fetal brain and colon.