|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human HIF1A ORF mammalian expression plasmid, C-GFPSpark tag||HG11977-ACG|
|Human HIF1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11977-ACR|
|Human HIF1A ORF mammalian expression plasmid, N-GFPSpark tag||HG11977-ANG|
|Human HIF1A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11977-ANR|
|Human HIF1A ORF mammalian expression plasmid, C-Flag tag||HG11977-CF|
|Human HIF1A ORF mammalian expression plasmid, C-His tag||HG11977-CH|
|Human HIF1A ORF mammalian expression plasmid, C-Myc tag||HG11977-CM|
|Human HIF1A ORF mammalian expression plasmid, C-HA tag||HG11977-CY|
|Human HIF1A Gene cDNA clone plasmid||HG11977-G|
|Human HIF1A ORF mammalian expression plasmid, N-Flag tag||HG11977-NF|
|Human HIF1A ORF mammalian expression plasmid, N-His tag||HG11977-NH|
|Human HIF1A ORF mammalian expression plasmid, N-Myc tag||HG11977-NM|
|Human HIF1A ORF mammalian expression plasmid, N-HA tag||HG11977-NY|
|Human HIF1A natural ORF mammalian expression plasmid||HG11977-UT|
|Learn more about expression Vectors|
HIF-1 alpha, also known as HIF1A, contains 1 basic helix-loop-helix (bHLH) domain, 1 PAC (PAS-associated C-terminal) domain and 2 PAS (PER-ARNT-SIM) domains. It is one of the two subunits of Hypoxia-inducible factor-1 (HIF1). HIF1 is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF-1 alpha is expressed in most tissues with highest levels in kidney and heart. It is overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, it activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. HIF-1 alpha binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300.