|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SLAMF7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11691-ACG|
|Human SLAMF7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11691-ACR|
|Human SLAMF7 ORF mammalian expression plasmid, C-Flag tag||HG11691-CF|
|Human SLAMF7 ORF mammalian expression plasmid, C-His tag||HG11691-CH|
|Human SLAMF7 ORF mammalian expression plasmid, C-Myc tag||HG11691-CM|
|Human SLAMF7 ORF mammalian expression plasmid, C-HA tag||HG11691-CY|
|Human SLAMF7 Gene cDNA clone plasmid||HG11691-G|
|Human SLAMF7 ORF mammalian expression plasmid, Flag tag||HG11691-G-F|
|Human SLAMF7 ORF mammalian expression plasmid, N-Flag tag||HG11691-NF|
|Human SLAMF7 ORF mammalian expression plasmid, N-His tag||HG11691-NH|
|Human SLAMF7 ORF mammalian expression plasmid, N-Myc tag||HG11691-NM|
|Human SLAMF7 ORF mammalian expression plasmid, N-HA tag||HG11691-NY|
|Human SLAMF7 natural ORF mammalian expression plasmid||HG11691-UT|
|Learn more about expression Vectors|
SLAM family member 7 (SLAMF7), also known as CRACC, CD319, CD2-like receptor-activating cytotoxic cells, and CS1, is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. CS1 is a self ligand and homophilic interaction of CS1 regulates NK cell cytolytic activity. CRACC positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, CRACC can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.