|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TFPI ORF mammalian expression plasmid, C-GFPSpark tag||HG10564-ACG|
|Human TFPI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10564-ACR|
|Human TFPI ORF mammalian expression plasmid, C-Flag tag||HG10564-CF|
|Human TFPI ORF mammalian expression plasmid, C-His tag||HG10564-CH|
|Human TFPI ORF mammalian expression plasmid, C-Myc tag||HG10564-CM|
|Human TFPI ORF mammalian expression plasmid, C-HA tag||HG10564-CY|
|Human TFPI Gene cDNA clone plasmid||HG10564-M|
|Human TFPI ORF mammalian expression plasmid, Flag tag||HG10564-M-F|
|Human TFPI ORF mammalian expression plasmid, N-Flag tag||HG10564-NF|
|Human TFPI ORF mammalian expression plasmid, N-His tag||HG10564-NH|
|Human TFPI ORF mammalian expression plasmid, N-Myc tag||HG10564-NM|
|Human TFPI ORF mammalian expression plasmid, N-HA tag||HG10564-NY|
|Human TFPI natural ORF mammalian expression plasmid||HG10564-UT|
|Learn more about expression Vectors|
Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.