|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human EPHA7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11657-ACG|
|Human EPHA7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11657-ACR|
|Human EPHA7 ORF mammalian expression plasmid, C-Flag tag||HG11657-CF|
|Human EPHA7 ORF mammalian expression plasmid, C-His tag||HG11657-CH|
|Human EPHA7 ORF mammalian expression plasmid, C-Myc tag||HG11657-CM|
|Human EPHA7 ORF mammalian expression plasmid, C-HA tag||HG11657-CY|
|Human EPHA7 Gene cDNA clone plasmid||HG11657-M|
|Human EPHA7 ORF mammalian expression plasmid, N-Flag tag||HG11657-NF|
|Human EPHA7 ORF mammalian expression plasmid, N-His tag||HG11657-NH|
|Human EPHA7 ORF mammalian expression plasmid, N-Myc tag||HG11657-NM|
|Human EPHA7 ORF mammalian expression plasmid, N-HA tag||HG11657-NY|
|Human EPHA7 natural ORF mammalian expression plasmid||HG11657-UT|
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Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.