|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human LTC4S ORF mammalian expression plasmid, C-GFPSpark tag||HG11622-ACG|
|Human LTC4S ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11622-ACR|
|Human LTC4S ORF mammalian expression plasmid, N-GFPSpark tag||HG11622-ANG|
|Human LTC4S ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11622-ANR|
|Human LTC4S ORF mammalian expression plasmid, C-Flag tag||HG11622-CF|
|Human LTC4S ORF mammalian expression plasmid, C-His tag||HG11622-CH|
|Human LTC4S ORF mammalian expression plasmid, C-Myc tag||HG11622-CM|
|Human LTC4S ORF mammalian expression plasmid, C-HA tag||HG11622-CY|
|Human LTC4S Gene cDNA clone plasmid||HG11622-M|
|Human LTC4S ORF mammalian expression plasmid, N-Flag tag||HG11622-NF|
|Human LTC4S ORF mammalian expression plasmid, N-His tag||HG11622-NH|
|Human LTC4S ORF mammalian expression plasmid, N-Myc tag||HG11622-NM|
|Human LTC4S ORF mammalian expression plasmid, N-HA tag||HG11622-NY|
|Human LTC4S natural ORF mammalian expression plasmid||HG11622-UT|
|Learn more about expression Vectors|
Leukotriene C4 synthase, also known as LTC4 synthase, Leukotriene-C(4) synthase, and LTC4S, is a multi-pass membrane protein which belongs to the MAPEG family. LTC4S is detected in lung, platelets and the myelogenous leukemia cell line KG-1 (at protein level). LTC4S activity is present in eosinophils, basophils, mast cells, certain phagocytic mononuclear cells, endothelial cells, vascular smooth muscle cells and platelets. LTC4S is essential for the production of cysteinyl leukotrienes (Cys-LT), critical mediators in asthma. Mutagenic analysis of the conjugation function of human LTC4S has identified R51 and Y93 as critical for acid and base catalysis of LTA4 and reduced glutathione, respectively. A comparison across species for proteins that possess LTC4S activity reveals conservation of both of these residues, whereas R51 is absent in the FLAP molecules. Thus, within the glutathione S-transferase superfamily of genes, alignment of specific residues allows the separation of LTC4S family members from their most structurally similar counterparts, the FLAP molecules. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.