|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ADAM15 ORF mammalian expression plasmid, C-GFPSpark tag||HG10517-ACG|
|Human ADAM15 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10517-ACR|
|Human ADAM15 ORF mammalian expression plasmid, C-Flag tag||HG10517-CF|
|Human ADAM15 ORF mammalian expression plasmid, C-His tag||HG10517-CH|
|Human ADAM15 ORF mammalian expression plasmid, C-Myc tag||HG10517-CM|
|Human ADAM15 ORF mammalian expression plasmid, C-HA tag||HG10517-CY|
|Human ADAM15 Gene cDNA clone plasmid||HG10517-M|
|Human ADAM15 ORF mammalian expression plasmid, N-Flag tag||HG10517-NF|
|Human ADAM15 ORF mammalian expression plasmid, N-His tag||HG10517-NH|
|Human ADAM15 ORF mammalian expression plasmid, N-Myc tag||HG10517-NM|
|Human ADAM15 ORF mammalian expression plasmid, N-HA tag||HG10517-NY|
|Human ADAM15 natural ORF mammalian expression plasmid||HG10517-UT|
|Learn more about expression Vectors|
ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell types. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression.