|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ESM1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11578-ACG|
|Human ESM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11578-ACR|
|Human ESM1 ORF mammalian expression plasmid, C-Flag tag||HG11578-CF|
|Human ESM1 ORF mammalian expression plasmid, C-His tag||HG11578-CH|
|Human ESM1 ORF mammalian expression plasmid, C-Myc tag||HG11578-CM|
|Human ESM1 ORF mammalian expression plasmid, C-HA tag||HG11578-CY|
|Human ESM1 Gene cDNA clone plasmid||HG11578-M|
|Human ESM1 ORF mammalian expression plasmid, N-Flag tag||HG11578-NF|
|Human ESM1 ORF mammalian expression plasmid, N-His tag||HG11578-NH|
|Human ESM1 ORF mammalian expression plasmid, N-Myc tag||HG11578-NM|
|Human ESM1 ORF mammalian expression plasmid, N-HA tag||HG11578-NY|
|Human ESM1 natural ORF mammalian expression plasmid||HG11578-UT|
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ESM1 is a secreted protein which is produced by adipocytes. It has been noticed that ESM1 may play some role in obesity-associated vascular disease since circulating ESM-1 levels are reduced in the overweight and obese. ESM1 is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of ESM1 gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. Recently, ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF or FGF-2. In hypervascularized cancers, overexpression of endocan has been detected by immunohistochemistry using monoclonal antibodies against ESM1.