|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human BID ORF mammalian expression plasmid, C-GFPSpark tag||HG10468-ACG|
|Human BID ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10468-ACR|
|Human BID ORF mammalian expression plasmid, N-GFPSpark tag||HG10468-ANG|
|Human BID ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10468-ANR|
|Human BID ORF mammalian expression plasmid, C-Flag tag||HG10468-CF|
|Human BID ORF mammalian expression plasmid, C-His tag||HG10468-CH|
|Human BID ORF mammalian expression plasmid, C-Myc tag||HG10468-CM|
|Human BID ORF mammalian expression plasmid, C-HA tag||HG10468-CY|
|Human BID Gene cDNA clone plasmid||HG10468-M|
|Human BID natural ORF mammalian expression plasmid||HG10468-M-N|
|Human BID ORF mammalian expression plasmid, N-Flag tag||HG10468-NF|
|Human BID ORF mammalian expression plasmid, N-His tag||HG10468-NH|
|Human BID ORF mammalian expression plasmid, N-Myc tag||HG10468-NM|
|Human BID ORF mammalian expression plasmid, N-HA tag||HG10468-NY|
|Human BID natural ORF mammalian expression plasmid||HG10468-UT|
|Learn more about expression Vectors|
The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.