|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ICOSLG ORF mammalian expression plasmid, C-GFPSpark tag||HG11559-ACG|
|Human ICOSLG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11559-ACR|
|Human ICOSLG ORF mammalian expression plasmid, C-Flag tag||HG11559-CF|
|Human ICOSLG ORF mammalian expression plasmid, C-His tag||HG11559-CH|
|Human ICOSLG ORF mammalian expression plasmid, C-Myc tag||HG11559-CM|
|Human ICOSLG ORF mammalian expression plasmid, C-HA tag||HG11559-CY|
|Human ICOSLG ORF mammalian expression plasmid, N-Flag tag||HG11559-NF|
|Human ICOSLG ORF mammalian expression plasmid, N-His tag||HG11559-NH|
|Human ICOSLG ORF mammalian expression plasmid, N-Myc tag||HG11559-NM|
|Human ICOSLG ORF mammalian expression plasmid, N-HA tag||HG11559-NY|
|Human ICOSLG natural ORF mammalian expression plasmid||HG11559-UT|
|Learn more about expression Vectors|
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.