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Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged

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PTPN12cDNA Clone Product Information
Gene_bank_ref_id:NM_002835.3
cDNA Size:2343
cDNA Description:ORF Clone of Homo sapiens protein tyrosine phosphatase, non-receptor type 12 DNA.
Gene Synonym:PTPG1, PTP-PEST, PTPN12
Species:Human
Vector:pCMV3-C-Myc
Restriction Site:
Tag Sequence:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-Myc Vector Information
 
Vector Name pCMV3-C-Myc
Vector Size 6164bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag Myc
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-Myc Physical Map

Schematic of pCMV3-C-Myc Multiple Cloning Sites

Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged on other vectors
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG11556-ACG$345
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG11556-ACR$345
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG11556-ANG$345
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG11556-ANR$345
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG11556-CF$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG11556-CH$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG11556-CM$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG11556-CY$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone)HG11556-M$195
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG11556-M-F$445
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11556-M-N$445
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG11556-NF$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG11556-NH$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG11556-NM$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG11556-NY$315
Human PTPN12 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11556-UT$315
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Background

PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein–protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.

References
  • Garton AJ. et al., 1997, Oncogene. 15 (8): 877-85.
  • Lin Yi. et al., 2003, Am J Physiol Heart Circ. 285 (2): H710-21.
  • Takekawa M. et al., 1994, FEBS Lett. 339 (3): 222-8.
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    Catalog:HG11556-CM
    List Price: $315.00  (Save $0.00)
    Price:$315.00      [How to order]
    Availability2-3 weeks
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