|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TAOK3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11547-ACG|
|Human TAOK3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11547-ACR|
|Human TAOK3 ORF mammalian expression plasmid, C-Flag tag||HG11547-CF|
|Human TAOK3 ORF mammalian expression plasmid, C-His tag||HG11547-CH|
|Human TAOK3 ORF mammalian expression plasmid, C-Myc tag||HG11547-CM|
|Human TAOK3 ORF mammalian expression plasmid, C-HA tag||HG11547-CY|
|Human TAOK3 Gene cDNA clone plasmid||HG11547-M|
|Human TAOK3 ORF mammalian expression plasmid, N-Flag tag||HG11547-NF|
|Human TAOK3 ORF mammalian expression plasmid, N-His tag||HG11547-NH|
|Human TAOK3 ORF mammalian expression plasmid, N-Myc tag||HG11547-NM|
|Human TAOK3 ORF mammalian expression plasmid, N-HA tag||HG11547-NY|
|Human TAOK3 natural ORF mammalian expression plasmid||HG11547-UT|
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Serine/threonine-protein kinase TAO3, also known as cutaneous T-cell lymphoma-associated antigen HD-CL-09, CTCL-associated antigen HD-CL-09, Dendritic cell-derived protein kinase, JNK / SAPK-inhibitory kinase, Jun kinase-inhibitory kinase, Kinase from chicken homolog A, Thousand and one amino acid protein 3, JIK, TAOK3 and MAP3K18, is cytoplasm and peripheral membrane protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Protein kinases are pivotal regulators of cell signaling that modulate each other's functions and activities through site-specific phosphorylation events. TAOK3 / JIK contains one protein kinase domain. TAOK3 / JIK is ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. TAOK3 / JIK inhibits the basal activity of Jun kinase. It is negatively regulated by epidermal growth factor (EGF). When overexpressed, TAOK3 / JIK may activate ERK1 / ERK2 and JNK / SAPK.