|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human VSIG2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11543-ACG|
|Human VSIG2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11543-ACR|
|Human VSIG2 ORF mammalian expression plasmid, C-Flag tag||HG11543-CF|
|Human VSIG2 ORF mammalian expression plasmid, C-His tag||HG11543-CH|
|Human VSIG2 ORF mammalian expression plasmid, C-Myc tag||HG11543-CM|
|Human VSIG2 ORF mammalian expression plasmid, C-HA tag||HG11543-CY|
|Human VSIG2 Gene cDNA clone plasmid||HG11543-M|
|Human VSIG2 ORF mammalian expression plasmid, N-Flag tag||HG11543-NF|
|Human VSIG2 ORF mammalian expression plasmid, N-His tag||HG11543-NH|
|Human VSIG2 ORF mammalian expression plasmid, N-Myc tag||HG11543-NM|
|Human VSIG2 ORF mammalian expression plasmid, N-HA tag||HG11543-NY|
|Human VSIG2 natural ORF mammalian expression plasmid||HG11543-UT|
|Learn more about expression Vectors|
V-set and immunoglobulin domain-containing protein 2, also known as cortical thymocyte-like protein, CT-like protein and VSIG2, is a single-pass type I membrane protein which contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. VSIG2 is highly expressed in stomach, colon, prostate, trachea and thyroid glands and weakly in bladder and lung. V-set domains are Ig-like domains resembling the antibody variable domain. V-set domains are found in diverse protein families, including immunoglobulin light and heavy chains; in several T-cell receptors such as CD2 (Cluster of Differentiation 2), CD4, CD80, and CD86; in myelin membrane adhesion molecules; in junction adhesion molecules (JAM); in tyrosine-protein kinase receptors; and in the programmed cell death protein 1 (PD1).