|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CST4 ORF mammalian expression plasmid, C-GFPSpark tag||HG11542-ACG|
|Human CST4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11542-ACR|
|Human CST4 ORF mammalian expression plasmid, C-Flag tag||HG11542-CF|
|Human CST4 ORF mammalian expression plasmid, C-His tag||HG11542-CH|
|Human CST4 ORF mammalian expression plasmid, C-Myc tag||HG11542-CM|
|Human CST4 ORF mammalian expression plasmid, C-HA tag||HG11542-CY|
|Human CST4 Gene cDNA clone plasmid||HG11542-M|
|Human CST4 natural ORF mammalian expression plasmid||HG11542-M-N|
|Human CST4 ORF mammalian expression plasmid, N-Flag tag||HG11542-NF|
|Human CST4 ORF mammalian expression plasmid, N-His tag||HG11542-NH|
|Human CST4 ORF mammalian expression plasmid, N-Myc tag||HG11542-NM|
|Human CST4 ORF mammalian expression plasmid, N-HA tag||HG11542-NY|
|Human CST4 natural ORF mammalian expression plasmid||HG11542-UT|
|Learn more about expression Vectors|
Cystatin-S, also known as Cystatin-4, Salivary acidic protein 1, Cystatin-SA-III and CST4, is a secreted protein which belongs to the cystatin family. Cystatin-4 / CST4 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in saliva, tears, urine and seminal fluid. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Cystatin-4 / CST4 strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Cystatin-4 / CST4 is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function.