|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human FCRL1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11536-ACG|
|Human FCRL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11536-ACR|
|Human FCRL1 ORF mammalian expression plasmid, C-Flag tag||HG11536-CF|
|Human FCRL1 ORF mammalian expression plasmid, C-His tag||HG11536-CH|
|Human FCRL1 ORF mammalian expression plasmid, C-Myc tag||HG11536-CM|
|Human FCRL1 ORF mammalian expression plasmid, C-HA tag||HG11536-CY|
|Human FCRL1 Gene cDNA clone plasmid||HG11536-M|
|Human FCRL1 ORF mammalian expression plasmid, Flag tag||HG11536-M-F|
|Human FCRL1 ORF mammalian expression plasmid, N-Flag tag||HG11536-NF|
|Human FCRL1 ORF mammalian expression plasmid, N-His tag||HG11536-NH|
|Human FCRL1 ORF mammalian expression plasmid, N-Myc tag||HG11536-NM|
|Human FCRL1 ORF mammalian expression plasmid, N-HA tag||HG11536-NY|
|Human FCRL1 natural ORF mammalian expression plasmid||HG11536-UT|
|Learn more about expression Vectors|
Fc receptor-like protein 1, also known as FcR-like protein 1, Fc receptor homolog 1, IFGP family protein 1, Immune receptor translocation-associated protein 5 and FCRL1, is a single-pass type I membrane protein which contains three Ig-like C2-type (immunoglobulin-like) domains. It is a cell-surface membrane protein belonging to FCRL family and is preferentially expressed on B cells. FCRL1 is primarily expressed in secondary lymphoid tissues by mature subsets of B cells. It is detected in spleen, lymph node, heart, skeletal muscle, kidney, liver and placenta. FCRL1 is specifically expressed by mature B lineage cells with higher expression in naive versus memory B cells (at protein level). Human Fc receptor-like molecules (FCRL1, FCRL2, FCRL3, FCRL4, FCRL5) have tyrosine-based immunoregulatory potential and are expressed by B-lineage subpopulations. FCRL1 may function as an activating coreceptor in B cells. It may also function in B cells activation and differentiation.