|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human F13B ORF mammalian expression plasmid, C-GFPSpark tag||HG11510-ACG|
|Human F13B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11510-ACR|
|Human F13B ORF mammalian expression plasmid, C-Flag tag||HG11510-CF|
|Human F13B ORF mammalian expression plasmid, C-His tag||HG11510-CH|
|Human F13B ORF mammalian expression plasmid, C-Myc tag||HG11510-CM|
|Human F13B ORF mammalian expression plasmid, C-HA tag||HG11510-CY|
|Human F13B Gene cDNA clone plasmid||HG11510-M|
|Human F13B ORF mammalian expression plasmid, N-Flag tag||HG11510-NF|
|Human F13B ORF mammalian expression plasmid, N-His tag||HG11510-NH|
|Human F13B ORF mammalian expression plasmid, N-Myc tag||HG11510-NM|
|Human F13B ORF mammalian expression plasmid, N-HA tag||HG11510-NY|
|Human F13B natural ORF mammalian expression plasmid||HG11510-UT|
|Learn more about expression Vectors|
Coagulation factor XIII B chain, also known as Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain and F13B, is a secreted protein which contains 10 Sushi ( CCP / SCR ) domains. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Factor XIII acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Defects in F13B are the cause of factor XIII subunit B deficiency ( FA13BD ) which is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.