|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TNFRII ORF mammalian expression plasmid, C-GFPSpark tag||HG10417-ACG|
|Human TNFRII ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10417-ACR|
|Human TNFRII ORF mammalian expression plasmid, C-Flag tag||HG10417-CF|
|Human TNFRII ORF mammalian expression plasmid, C-His tag||HG10417-CH|
|Human TNFRII ORF mammalian expression plasmid, C-Myc tag||HG10417-CM|
|Human TNFRII ORF mammalian expression plasmid, C-HA tag||HG10417-CY|
|Human TNFRII Gene cDNA clone plasmid||HG10417-M|
|Human TNFRII ORF mammalian expression plasmid, N-Flag tag||HG10417-NF|
|Human TNFRII ORF mammalian expression plasmid, N-His tag||HG10417-NH|
|Human TNFRII ORF mammalian expression plasmid, N-Myc tag||HG10417-NM|
|Human TNFRII ORF mammalian expression plasmid, N-HA tag||HG10417-NY|
|Human TNFRII natural ORF mammalian expression plasmid||HG10417-UT|
|Learn more about expression Vectors|
Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.