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Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-tagged

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CES2cDNA Clone Product Information
Gene_bank_ref_id:NM_003869.4
cDNA Size:1872
cDNA Description:ORF Clone of Homo sapiens carboxylesterase 2 (intestine, liver) DNA.
Gene Synonym:iCE, CE-2, PCE-2, CES2A1
Species:Human
Vector:pCMV3-N-Myc
Restriction Site:
Tag Sequence:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-N-Myc Vector Information
 
Vector Name pCMV3-N-Myc
Vector Size 6104bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag Myc
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-N-Myc Physical Map

Schematic of pCMV3-N-Myc Multiple Cloning Sites

Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-tagged on other vectors
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10380-ACG$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10380-ACR$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10380-ANG$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10380-ANR$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10380-CF$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10380-CH$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10380-CM$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10380-CY$315
Human CES2 Gene cDNA Clone (full-length ORF Clone)HG10380-M$195
Human CES2 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10380-M-F$395
Human CES2 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10380-M-N$395
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10380-NF$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10380-NH$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10380-NM$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10380-NY$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10380-UT$315
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Background

Carboxylesterase 2 (CES2) is a member of the carboxylesterase family and belongs to the multigene family. Carboxylesterase 2 is responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and beroin. It also serves to hydrolyze long-chain fatty acid esters and thioesters. It is speculated that carboxylesterases may play a role in lipid metabolism and the blood-brain barrier system and together with isform 1, are a serine esterase involved in both drug metabolism and activation. Human carboxylesterase 2 is commonly expressed in tumor tissues and irinotecan, a topoisomerase I inhibitor commonly used in the treatment of many solid tumors.

References
  • Imai T. et al. (2006) Human carboxylesterase isozymes: catalytic properties and rational drug design. Drug metab pharmacokinet. 21 (3): 173-85.
  • Guang Xu, et al. (2002) Human carboxylesterase 2 is commonly expressed in tumor tissue and is correlated with activation of irinotecan. Clin Cancer Res. 8: 2605.
  • Zhang, et al. (2002) Comprehensive Evaluation of Carboxylesterase-2 Expression in Normal Human Tissues Using Tissue Array Analysis. Applied Immunohistochemistry & Molecular Morphology. 10 (4): 374-80.
  • Size / Price
    Catalog:HG10380-NM
    List Price: $315.00  (Save $0.00)
    Price:$315.00      [How to order]
    Availability2-3 weeks
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