|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human RBP4 ORF mammalian expression plasmid, C-GFPSpark tag||HG10354-ACG|
|Human RBP4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10354-ACR|
|Human RBP4 ORF mammalian expression plasmid, C-Flag tag||HG10354-CF|
|Human RBP4 ORF mammalian expression plasmid, C-His tag||HG10354-CH|
|Human RBP4 ORF mammalian expression plasmid, C-Myc tag||HG10354-CM|
|Human RBP4 ORF mammalian expression plasmid, C-HA tag||HG10354-CY|
|Human RBP4 Gene cDNA clone plasmid||HG10354-M|
|Human RBP4 ORF mammalian expression plasmid, N-Flag tag||HG10354-NF|
|Human RBP4 ORF mammalian expression plasmid, N-His tag||HG10354-NH|
|Human RBP4 ORF mammalian expression plasmid, N-Myc tag||HG10354-NM|
|Human RBP4 ORF mammalian expression plasmid, N-HA tag||HG10354-NY|
|Human RBP4 natural ORF mammalian expression plasmid||HG10354-UT|
|Learn more about expression Vectors|
Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).