|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ICOS ORF mammalian expression plasmid, C-GFPSpark tag||HG10344-ACG|
|Human ICOS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10344-ACR|
|Human ICOS ORF mammalian expression plasmid, C-Flag tag||HG10344-CF|
|Human ICOS ORF mammalian expression plasmid, C-His tag||HG10344-CH|
|Human ICOS ORF mammalian expression plasmid, C-Myc tag||HG10344-CM|
|Human ICOS ORF mammalian expression plasmid, C-HA tag||HG10344-CY|
|Human ICOS Gene cDNA clone plasmid||HG10344-M|
|Human ICOS ORF mammalian expression plasmid, N-Flag tag||HG10344-NF|
|Human ICOS ORF mammalian expression plasmid, N-His tag||HG10344-NH|
|Human ICOS ORF mammalian expression plasmid, N-Myc tag||HG10344-NM|
|Human ICOS ORF mammalian expression plasmid, N-HA tag||HG10344-NY|
|Human ICOS natural ORF mammalian expression plasmid||HG10344-UT|
|Learn more about expression Vectors|
Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T−B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS is more potent in the induction of IL-10 production, a cytokine important for suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provides key second signals that can regulate the activation, inhibition and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested of being involved in the development of airway inflammation and airway hyperresponsiveness.