|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SDC1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11429-ACG|
|Human SDC1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11429-ACR|
|Human SDC1 ORF mammalian expression plasmid, C-Flag tag||HG11429-CF|
|Human SDC1 ORF mammalian expression plasmid, C-His tag||HG11429-CH|
|Human SDC1 ORF mammalian expression plasmid, C-Myc tag||HG11429-CM|
|Human SDC1 ORF mammalian expression plasmid, C-HA tag||HG11429-CY|
|Human SDC1 Gene cDNA clone plasmid||HG11429-M|
|Human SDC1 ORF mammalian expression plasmid, His tag||HG11429-M-H|
|Human SDC1 ORF mammalian expression plasmid, N-Flag tag||HG11429-NF|
|Human SDC1 ORF mammalian expression plasmid, N-His tag||HG11429-NH|
|Human SDC1 ORF mammalian expression plasmid, N-Myc tag||HG11429-NM|
|Human SDC1 ORF mammalian expression plasmid, N-HA tag||HG11429-NY|
|Human SDC1 natural ORF mammalian expression plasmid||HG11429-UT|
|Learn more about expression Vectors|
Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.