|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Rhesus HSPA8 ORF mammalian expression plasmid, C-GFPSpark tag||CG90471-ACG|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90471-ACR|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-Flag tag||CG90471-CF|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-His tag||CG90471-CH|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-Myc tag||CG90471-CM|
|Rhesus HSPA8 ORF mammalian expression plasmid, C-HA tag||CG90471-CY|
|Rhesus HSPA8 Gene cDNA clone plasmid||CG90471-G|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-Flag tag||CG90471-NF|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-His tag||CG90471-NH|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-Myc tag||CG90471-NM|
|Rhesus HSPA8 ORF mammalian expression plasmid, N-HA tag||CG90471-NY|
|Rhesus HSPA8 natural ORF mammalian expression plasmid||CG90471-UT|
|Learn more about expression Vectors|
HSPA8, also known as HSC70, is a member of the heat shock protein family due to homology with other heat shock proteins. The heat shock protein 70 family is comprised by both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 belongs to the heat-shock cognate subgroup. Members of the human heat-shock protein multigene family have several highly conserved proteins with structural and functional properties in common, but vary in the extent of their inducibility in response to metabolic stress. HSPA8 is constitutively expressed and performs functions related to normal cellular processes. This protein binds to nascent polypeptides to facilitate correct protein folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. HSPA8 acts as a repressor of transcriptional activation. It inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. It also is a ATPase that works with auxilin to remove clathrin coated vesicles. In neurons, synaptojanin is also an important protein involved in vesicle uncoating.