|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human Pin1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10282-ACG|
|Human Pin1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10282-ACR|
|Human Pin1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10282-ANG|
|Human Pin1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10282-ANR|
|Human Pin1 ORF mammalian expression plasmid, C-Flag tag||HG10282-CF|
|Human Pin1 ORF mammalian expression plasmid, C-His tag||HG10282-CH|
|Human Pin1 ORF mammalian expression plasmid, C-Myc tag||HG10282-CM|
|Human Pin1 ORF mammalian expression plasmid, C-HA tag||HG10282-CY|
|Human Pin1 Gene cDNA clone plasmid||HG10282-M|
|Human Pin1 ORF mammalian expression plasmid, Flag tag||HG10282-M-F|
|Human Pin1 ORF mammalian expression plasmid, N-Flag tag||HG10282-NF|
|Human Pin1 ORF mammalian expression plasmid, N-His tag||HG10282-NH|
|Human Pin1 ORF mammalian expression plasmid, N-Myc tag||HG10282-NM|
|Human Pin1 ORF mammalian expression plasmid, N-HA tag||HG10282-NY|
|Human Pin1 natural ORF mammalian expression plasmid||HG10282-UT|
|Learn more about expression Vectors|
Peptidyl-prolyl cis-trans isomerase Pin1, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Rotamase Pin1 and PIN1, peptidyl-prolyl cis/trans isomerase (PPIase), is a nucleus protein. PIN1 is a peptidyl-prolyl isomerase that can alter the conformation of phosphoproteins and so affect protein function and/or stability. PIN1 regulates a number of proteins important for cell-cycle progression and is presumed to operate as a molecular timer of this important process. PIN1 is an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. PIN1 displays a preference for an acidic residue N-terminal to the isomerized proline bond. Alterations in the level of PIN1 can influence hyperproliferative diseases such as cancer. PIN1 has been implicated in multiple aspects of cell cycle regulation. It has been suggested that PIN1 function is required for both normal mitotic progression and reentry into the cell cycle from quiescence. PIN1 is also a target of several oncogenic pathways and is overexpressed in human breast cancer. Its overexpression can lead to upregulation of cyclin-D1 and transformation of breast epithelial cells in collaboration with the oncogenic pathways. PIN1 plays a pivotal role in breast development and may be a promising new anticancer target. Pin1 activity regulates the outcome of proline-directed kinase (e.g. MAPK, CDK or GSK3) signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell survival. Recent data also implicate Pin1 as playing an important role in immune responses, at least in part by increasing the stability of cytokine mRNAs by influencing the protein complexes to which they bind.