|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ENPP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11308-ACG|
|Human ENPP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11308-ACR|
|Human ENPP2 ORF mammalian expression plasmid, C-Flag tag||HG11308-CF|
|Human ENPP2 ORF mammalian expression plasmid, C-His tag||HG11308-CH|
|Human ENPP2 ORF mammalian expression plasmid, C-Myc tag||HG11308-CM|
|Human ENPP2 ORF mammalian expression plasmid, C-HA tag||HG11308-CY|
|Human ENPP2 Gene cDNA clone plasmid||HG11308-M|
|Human ENPP2 ORF mammalian expression plasmid, Flag tag||HG11308-M-F|
|Human ENPP2 ORF mammalian expression plasmid, N-Flag tag||HG11308-NF|
|Human ENPP2 ORF mammalian expression plasmid, N-His tag||HG11308-NH|
|Human ENPP2 ORF mammalian expression plasmid, N-Myc tag||HG11308-NM|
|Human ENPP2 ORF mammalian expression plasmid, N-HA tag||HG11308-NY|
|Human ENPP2 natural ORF mammalian expression plasmid||HG11308-UT|
|Learn more about expression Vectors|
ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.