|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TLR3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10190-ACG|
|Human TLR3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10190-ACR|
|Human TLR3 ORF mammalian expression plasmid, N-GFPSpark tag||HG10190-ANG|
|Human TLR3 ORF mammalian expression plasmid, C-Flag tag||HG10190-CF|
|Human TLR3 ORF mammalian expression plasmid, C-His tag||HG10190-CH|
|Human TLR3 ORF mammalian expression plasmid, C-Myc tag||HG10190-CM|
|Human TLR3 ORF mammalian expression plasmid, C-HA tag||HG10190-CY|
|Human TLR3 Gene cDNA clone plasmid||HG10190-G|
|Human TLR3 ORF mammalian expression plasmid, N-Flag tag||HG10190-NF|
|Human TLR3 ORF mammalian expression plasmid, N-His tag||HG10190-NH|
|Human TLR3 ORF mammalian expression plasmid, N-Myc tag||HG10190-NM|
|Human TLR3 ORF mammalian expression plasmid, N-HA tag||HG10190-NY|
|Human TLR3 natural ORF mammalian expression plasmid||HG10190-UT|
|Learn more about expression Vectors|
Toll-like receptor 3 (TLR3) also known as CD283 (cluster of differentiation 283) is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. TLR3/CD283 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses.