|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human LTF ORF mammalian expression plasmid, C-GFPSpark tag||HG11096-ACG|
|Human LTF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11096-ACR|
|Human LTF ORF mammalian expression plasmid, C-Flag tag||HG11096-CF|
|Human LTF ORF mammalian expression plasmid, C-His tag||HG11096-CH|
|Human LTF ORF mammalian expression plasmid, C-Myc tag||HG11096-CM|
|Human LTF ORF mammalian expression plasmid, C-HA tag||HG11096-CY|
|Human LTF Gene cDNA clone plasmid||HG11096-M|
|Human LTF natural ORF mammalian expression plasmid||HG11096-M-N|
|Human LTF ORF mammalian expression plasmid, N-Flag tag||HG11096-NF|
|Human LTF ORF mammalian expression plasmid, N-His tag||HG11096-NH|
|Human LTF ORF mammalian expression plasmid, N-Myc tag||HG11096-NM|
|Human LTF ORF mammalian expression plasmid, N-HA tag||HG11096-NY|
|Human LTF natural ORF mammalian expression plasmid||HG11096-UT|
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Lactotransferrin, also known as Lactoferrin, Talalactoferrin and LTF, is a secreted protein which belongs to the transferrin family. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and lactoferroxin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin / LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin / LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin / LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin / LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.