|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human RSPO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11083-ACG|
|Human RSPO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11083-ACR|
|Human RSPO1 ORF mammalian expression plasmid, C-Flag tag||HG11083-CF|
|Human RSPO1 ORF mammalian expression plasmid, C-His tag||HG11083-CH|
|Human RSPO1 ORF mammalian expression plasmid, C-Myc tag||HG11083-CM|
|Human RSPO1 ORF mammalian expression plasmid, C-HA tag||HG11083-CY|
|Human RSPO1 Gene cDNA clone plasmid||HG11083-M|
|Human RSPO1 ORF mammalian expression plasmid, N-Flag tag||HG11083-NF|
|Human RSPO1 ORF mammalian expression plasmid, N-His tag||HG11083-NH|
|Human RSPO1 ORF mammalian expression plasmid, N-Myc tag||HG11083-NM|
|Human RSPO1 ORF mammalian expression plasmid, N-HA tag||HG11083-NY|
|Human RSPO1 natural ORF mammalian expression plasmid||HG11083-UT|
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RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.