|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Ferret PRMT6 ORF mammalian expression plasmid, C-GFPSpark tag||FG60131-ACG|
|Ferret PRMT6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||FG60131-ACR|
|Ferret PRMT6 ORF mammalian expression plasmid, N-GFPSpark tag||FG60131-ANG|
|Ferret PRMT6 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||FG60131-ANR|
|Ferret PRMT6 ORF mammalian expression plasmid, C-Flag tag||FG60131-CF|
|Ferret PRMT6 ORF mammalian expression plasmid, C-His tag||FG60131-CH|
|Ferret PRMT6 ORF mammalian expression plasmid, C-Myc tag||FG60131-CM|
|Ferret PRMT6 ORF mammalian expression plasmid, C-HA tag||FG60131-CY|
|Ferret PRMT6 Gene cDNA clone plasmid||FG60131-G|
|Ferret PRMT6 ORF mammalian expression plasmid, N-Flag tag||FG60131-NF|
|Ferret PRMT6 ORF mammalian expression plasmid, N-His tag||FG60131-NH|
|Ferret PRMT6 ORF mammalian expression plasmid, N-Myc tag||FG60131-NM|
|Ferret PRMT6 ORF mammalian expression plasmid, N-HA tag||FG60131-NY|
|Ferret PRMT6 natural ORF mammalian expression plasmid||FG60131-UT|
|Learn more about expression Vectors|
Protein arginine N-methyltransferase 6, also known as Histone-arginine N-methyltransferase PRMT6, PRMT6, and HRMT1L6, is a member of the protein arginine N-methyltransferase family and PRMT6 subfamily. PRMT6 is highly expressed in kidney and testes. PRMT6 is known to catalyze the generation of asymmetric dimethylarginine in polypeptides. It has been implicated in human immunodeficiency virus pathogenesis, DNA repair, and transcriptional regulation. PRMT6 is known to methylate histone H3 Arg-2 (H3R2), and this negatively regulates the lysine methylation of H3K4 resulting in gene repression. PRMT6 plays a key role in coupling process by functioning as a transcriptional coactivator that can regulate alternative splicing. PRMT6 coactivates the progesterone, glucocorticoid and oestrogen receptors in luciferase reporter assays in a hormone-dependent manner. Small interfering RNA (siRNA) oligonucleotide duplex knockdown of PRMT6 disrupts oestrogen-stimulated transcription of endogenous GREB1 and progesterone receptor in MCF-7 breast cancer cells. Neutralizing the activity of PRMT6 could inhibit tumor progression and may be of cancer therapeutic significance.