|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CD5 ORF mammalian expression plasmid, C-GFPSpark tag||HG11027-ACG|
|Human CD5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11027-ACR|
|Human CD5 ORF mammalian expression plasmid, C-Flag tag||HG11027-CF|
|Human CD5 ORF mammalian expression plasmid, C-His tag||HG11027-CH|
|Human CD5 ORF mammalian expression plasmid, C-Myc tag||HG11027-CM|
|Human CD5 ORF mammalian expression plasmid, C-HA tag||HG11027-CY|
|Human CD5 Gene cDNA clone plasmid||HG11027-M|
|Human CD5 ORF mammalian expression plasmid, N-Flag tag||HG11027-NF|
|Human CD5 ORF mammalian expression plasmid, N-His tag||HG11027-NH|
|Human CD5 ORF mammalian expression plasmid, N-Myc tag||HG11027-NM|
|Human CD5 ORF mammalian expression plasmid, N-HA tag||HG11027-NY|
|Human CD5 natural ORF mammalian expression plasmid||HG11027-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.