|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SERPINA7 ORF mammalian expression plasmid, C-GFPSpark tag||HG10993-ACG|
|Human SERPINA7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10993-ACR|
|Human SERPINA7 ORF mammalian expression plasmid, C-Flag tag||HG10993-CF|
|Human SERPINA7 ORF mammalian expression plasmid, C-His tag||HG10993-CH|
|Human SERPINA7 ORF mammalian expression plasmid, C-Myc tag||HG10993-CM|
|Human SERPINA7 ORF mammalian expression plasmid, C-HA tag||HG10993-CY|
|Human SERPINA7 ORF mammalian expression plasmid, N-Flag tag||HG10993-NF|
|Human SERPINA7 ORF mammalian expression plasmid, N-His tag||HG10993-NH|
|Human SERPINA7 ORF mammalian expression plasmid, N-Myc tag||HG10993-NM|
|Human SERPINA7 ORF mammalian expression plasmid, N-HA tag||HG10993-NY|
|Human SERPINA7 natural ORF mammalian expression plasmid||HG10993-UT|
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Thyroxine-binding globulin, also known as T4-binding globulin, Serpin A7 and TBG, is a secreted protein which belongs to the serpin family. TBG is synthesized primarily in the liver as a 54 kDa protein.TBG is genomically a serpin, although it has no inhibitory function like many other members of this class of proteins. TBG binds thyroid hormone in circulation. It is one of three proteins (along with transthyretin and albumin) responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3’-triiodothyronine (T3) in the bloodstream. Of these three proteins, TBG has the highest affinity for T4 and T3, but is present in the lowest concentration. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 and T3, TBG is rarely more than 25% saturated with its ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. TBG tests are sometimes used in finding the reason for elevated or diminished levels of thyroid hormone.