|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SERPINA10 ORF mammalian expression plasmid, C-GFPSpark tag||HG10992-ACG|
|Human SERPINA10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10992-ACR|
|Human SERPINA10 ORF mammalian expression plasmid, C-Flag tag||HG10992-CF|
|Human SERPINA10 ORF mammalian expression plasmid, C-His tag||HG10992-CH|
|Human SERPINA10 ORF mammalian expression plasmid, C-Myc tag||HG10992-CM|
|Human SERPINA10 ORF mammalian expression plasmid, C-HA tag||HG10992-CY|
|Human SERPINA10 Gene cDNA clone plasmid||HG10992-M|
|Human SERPINA10 natural ORF mammalian expression plasmid||HG10992-M-N|
|Human SERPINA10 ORF mammalian expression plasmid, N-Flag tag||HG10992-NF|
|Human SERPINA10 ORF mammalian expression plasmid, N-His tag||HG10992-NH|
|Human SERPINA10 ORF mammalian expression plasmid, N-Myc tag||HG10992-NM|
|Human SERPINA10 ORF mammalian expression plasmid, N-HA tag||HG10992-NY|
|Human SERPINA10 natural ORF mammalian expression plasmid||HG10992-UT|
|Learn more about expression Vectors|
Mouse protein Z-dependent protease inhibitor, also known as PZ-dependent protease inhibitor, SERPINA10 and ZPI, is a secreted protein which belongs to the serpin family. It is expressed by the liver and secreted in plasma. SERPINA10 / Serpin-A10 inhibits factor Xa activity in the presence of protein Z, calcium and phospholipid. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases (serine protease inhibitors).Over 1000 serpins have now been identified, these include 36 human proteins, as well as molecules in plants, fungi, bacteria, archaea and certain viruses. Serpins are the largest and most diverse family of protease inhibitors. Most serpins control proteolytic cascades, certain serpins do not inhibit enzymes, but instead perform diverse functions such as storage (ovalbumin, in egg white), hormone carriage proteins (thyroxine-binding globulin, cortisol-binding globulin) and tumor suppressor genes (maspin). Most inhibitory serpins target chymotrypsin-like serine proteases. These enzymes are defined by the presence of a nucleophilic serine residue in their catalytic site. Some serpins inhibit other classes of protease. A number of such serpins have been shown to target cysteine proteases. These enzymes differ from serine proteases in that they are defined by the presence of a nucleophilic cysteine residue, rather than a serine residue, in their catalytic site.