|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CCL8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10989-ACG|
|Human CCL8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10989-ACR|
|Human CCL8 ORF mammalian expression plasmid, C-Flag tag||HG10989-CF|
|Human CCL8 ORF mammalian expression plasmid, C-His tag||HG10989-CH|
|Human CCL8 ORF mammalian expression plasmid, C-Myc tag||HG10989-CM|
|Human CCL8 ORF mammalian expression plasmid, C-HA tag||HG10989-CY|
|Human CCL8 Gene cDNA clone plasmid||HG10989-M|
|Human CCL8 ORF mammalian expression plasmid, Flag tag||HG10989-M-F|
|Human CCL8 natural ORF mammalian expression plasmid||HG10989-M-N|
|Human CCL8 ORF mammalian expression plasmid, N-Flag tag||HG10989-NF|
|Human CCL8 ORF mammalian expression plasmid, N-His tag||HG10989-NH|
|Human CCL8 ORF mammalian expression plasmid, N-Myc tag||HG10989-NM|
|Human CCL8 ORF mammalian expression plasmid, N-HA tag||HG10989-NY|
|Human CCL8 natural ORF mammalian expression plasmid||HG10989-UT|
|Learn more about expression Vectors|
Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP-2), is a small cytokine belonging to the C-C chemokine family. CCL8 functions to activate different immune cells, including mast cells, eosinophils and basophils which are involved in allergic responses, monocytes, and T cells and NK cells which are involved in the inflammatory response. CCL8's ability achieves by binding to different cell surface receptors termed chemokine receptors including CCR1, CCR2B and CCR5. It has been reported that CCL8 is a potent inhibitor of HIV-1 by virtue of its binding to CCR5 which is one of the major co-receptors for HIV-1.