|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human KLRF1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10984-ACG|
|Human KLRF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10984-ACR|
|Human KLRF1 ORF mammalian expression plasmid, C-Flag tag||HG10984-CF|
|Human KLRF1 ORF mammalian expression plasmid, C-His tag||HG10984-CH|
|Human KLRF1 ORF mammalian expression plasmid, C-Myc tag||HG10984-CM|
|Human KLRF1 ORF mammalian expression plasmid, C-HA tag||HG10984-CY|
|Human KLRF1 Gene cDNA clone plasmid||HG10984-M|
|Human KLRF1 ORF mammalian expression plasmid, N-Flag tag||HG10984-NF|
|Human KLRF1 ORF mammalian expression plasmid, N-His tag||HG10984-NH|
|Human KLRF1 ORF mammalian expression plasmid, N-Myc tag||HG10984-NM|
|Human KLRF1 ORF mammalian expression plasmid, N-HA tag||HG10984-NY|
|Human KLRF1 natural ORF mammalian expression plasmid||HG10984-UT|
|Learn more about expression Vectors|
NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor which is expressed on nearly all natural killer cells and stimulates their cytoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y30F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp80, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-70 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.