|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human RENIN ORF mammalian expression plasmid, C-GFPSpark tag||HG10969-ACG|
|Human RENIN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10969-ACR|
|Human RENIN ORF mammalian expression plasmid, C-Flag tag||HG10969-CF|
|Human RENIN ORF mammalian expression plasmid, C-His tag||HG10969-CH|
|Human RENIN ORF mammalian expression plasmid, C-Myc tag||HG10969-CM|
|Human RENIN ORF mammalian expression plasmid, C-HA tag||HG10969-CY|
|Human RENIN Gene cDNA clone plasmid||HG10969-M|
|Human RENIN natural ORF mammalian expression plasmid||HG10969-M-N|
|Human RENIN ORF mammalian expression plasmid, N-Flag tag||HG10969-NF|
|Human RENIN ORF mammalian expression plasmid, N-His tag||HG10969-NH|
|Human RENIN ORF mammalian expression plasmid, N-Myc tag||HG10969-NM|
|Human RENIN ORF mammalian expression plasmid, N-HA tag||HG10969-NY|
|Human RENIN natural ORF mammalian expression plasmid||HG10969-UT|
|Learn more about expression Vectors|
Mouse Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.