|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Cynomolgus CD3E ORF mammalian expression plasmid, C-GFPSpark tag||CG90047-ACG|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90047-ACR|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-Flag tag||CG90047-CF|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-His tag||CG90047-CH|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-Myc tag||CG90047-CM|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-HA tag||CG90047-CY|
|Cynomolgus CD3E Gene cDNA clone plasmid||CG90047-G|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-Flag tag||CG90047-NF|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-His tag||CG90047-NH|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-Myc tag||CG90047-NM|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-HA tag||CG90047-NY|
|Cynomolgus CD3E natural ORF mammalian expression plasmid||CG90047-UT|
|Learn more about expression Vectors|
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.