|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human IL34 ORF mammalian expression plasmid, C-GFPSpark tag||HG10948-ACG|
|Human IL34 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10948-ACR|
|Human IL34 ORF mammalian expression plasmid, C-Flag tag||HG10948-CF|
|Human IL34 ORF mammalian expression plasmid, C-His tag||HG10948-CH|
|Human IL34 ORF mammalian expression plasmid, C-Myc tag||HG10948-CM|
|Human IL34 ORF mammalian expression plasmid, C-HA tag||HG10948-CY|
|Human IL34 Gene cDNA clone plasmid||HG10948-M|
|Human IL34 ORF mammalian expression plasmid, N-Flag tag||HG10948-NF|
|Human IL34 ORF mammalian expression plasmid, N-His tag||HG10948-NH|
|Human IL34 ORF mammalian expression plasmid, N-Myc tag||HG10948-NM|
|Human IL34 ORF mammalian expression plasmid, N-HA tag||HG10948-NY|
|Human IL34 natural ORF mammalian expression plasmid||HG10948-UT|
|Learn more about expression Vectors|
Interleukin 34 (IL-34), also known as uncharacterized protein C16 or f77 homolog, is a secreted cytokine that belongs to unknown cytokine family. IL-34 protein is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate and colon, and most abundant in the spleen. The colony-stimulating factor 1 receptor (CSF-1R) is identified as the receptor for IL-34. IL-34 increases growth or survival of immune cells known as monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.