|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human GDF-15 ORF mammalian expression plasmid, C-GFPSpark tag||HG10936-ACG|
|Human GDF-15 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10936-ACR|
|Human GDF-15 ORF mammalian expression plasmid, C-Flag tag||HG10936-CF|
|Human GDF-15 ORF mammalian expression plasmid, C-His tag||HG10936-CH|
|Human GDF-15 ORF mammalian expression plasmid, C-Myc tag||HG10936-CM|
|Human GDF-15 ORF mammalian expression plasmid, C-HA tag||HG10936-CY|
|Human GDF-15 Gene cDNA clone plasmid||HG10936-M|
|Human GDF-15 ORF mammalian expression plasmid, N-Flag tag||HG10936-NF|
|Human GDF-15 ORF mammalian expression plasmid, N-His tag||HG10936-NH|
|Human GDF-15 ORF mammalian expression plasmid, N-Myc tag||HG10936-NM|
|Human GDF-15 ORF mammalian expression plasmid, N-HA tag||HG10936-NY|
|Human GDF-15 natural ORF mammalian expression plasmid||HG10936-UT|
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Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-β superfamily, as a novel antihypertrophic regulatory factor in the heart. GDF-15 / GDF15 is not expressed in the normal adult heart but is induced in response to conditions that promote hypertrophy and dilated cardiomyopathy and it is expressed highly in liver. GDF-15 / GDF15 has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF-15 / GDF15 is synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein. GDF-15 / GDF15 overexpression arising from an expanded erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression.