|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TIMP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10934-ACG|
|Human TIMP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10934-ACR|
|Human TIMP1 ORF mammalian expression plasmid, C-Flag tag||HG10934-CF|
|Human TIMP1 ORF mammalian expression plasmid, C-His tag||HG10934-CH|
|Human TIMP1 ORF mammalian expression plasmid, C-Myc tag||HG10934-CM|
|Human TIMP1 ORF mammalian expression plasmid, C-HA tag||HG10934-CY|
|Human TIMP1 Gene cDNA clone plasmid||HG10934-M|
|Human TIMP1 ORF mammalian expression plasmid, Flag tag||HG10934-M-F|
|Human TIMP1 ORF mammalian expression plasmid, N-Flag tag||HG10934-NF|
|Human TIMP1 ORF mammalian expression plasmid, N-His tag||HG10934-NH|
|Human TIMP1 ORF mammalian expression plasmid, N-Myc tag||HG10934-NM|
|Human TIMP1 ORF mammalian expression plasmid, N-HA tag||HG10934-NY|
|Human TIMP1 natural ORF mammalian expression plasmid||HG10934-UT|
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TIMP metallopeptidase inhibitor 1, also known as TIMP-1/TIMP1, Collagenase inhibitor 16C8 fibroblast Erythroid-potentiating activity, TPA-S1TPA-induced proteinTissue inhibitor of metalloproteinases 1, is a natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. TIMP-1/TIMP1 is found in fetal and adult tissues. Highest levels are found in bone, lung, ovary and uterus. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. In addition to its inhibitory role against most of the known MMPs, the protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this protein encoding gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This encoding gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 is Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16.