|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CDH16 ORF mammalian expression plasmid, C-GFPSpark tag||HG10915-ACG|
|Human CDH16 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10915-ACR|
|Human CDH16 ORF mammalian expression plasmid, C-Flag tag||HG10915-CF|
|Human CDH16 ORF mammalian expression plasmid, C-His tag||HG10915-CH|
|Human CDH16 ORF mammalian expression plasmid, C-Myc tag||HG10915-CM|
|Human CDH16 ORF mammalian expression plasmid, C-HA tag||HG10915-CY|
|Human CDH16 Gene cDNA clone plasmid||HG10915-M|
|Human CDH16 ORF mammalian expression plasmid, Flag tag||HG10915-M-F|
|Human CDH16 ORF mammalian expression plasmid, N-Flag tag||HG10915-NF|
|Human CDH16 ORF mammalian expression plasmid, N-His tag||HG10915-NH|
|Human CDH16 ORF mammalian expression plasmid, N-Myc tag||HG10915-NM|
|Human CDH16 ORF mammalian expression plasmid, N-HA tag||HG10915-NY|
|Human CDH16 natural ORF mammalian expression plasmid||HG10915-UT|
|Learn more about expression Vectors|
KSP-Cadherin/Cadherin-16 is a member of the cadherin superfamily, calcium-dependent, membrane-associated glycoproteins. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. KSP-Cadherin/Cadherin-16 can be detected at later stages of tubulogenesis during human renal development and in the distal tubules of adult kidneys, no expression was found by immunohistochemistry or Western blot analysis in RCC tumour tissues and several RCC cell lines. However, despite the lack of protein expression, mRNA synthesis of KSP-Cadherin/Cadherin-16 could be detected by reverse transcriptase-polymerase chain reaction analysis in all RCC tissues and most of the RCC cell lines studied, although at a reduced level. The loss of KSP-Cadherin/Cadherin-16 protein was only observed in the malignant part of the tumour kidneys, whereas in the normal part of the affected kidneys KSP-Cadherin/Cadherin-16 expression was clearly detected. These results indicate a downregulation of Ksp-cadherin in RCC and suggest a role for this cell adhesion molecule in tumour suppression.