|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CCNE1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10902-ACG|
|Human CCNE1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10902-ACR|
|Human CCNE1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10902-ANG|
|Human CCNE1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10902-ANR|
|Human CCNE1 ORF mammalian expression plasmid, C-Flag tag||HG10902-CF|
|Human CCNE1 ORF mammalian expression plasmid, C-His tag||HG10902-CH|
|Human CCNE1 ORF mammalian expression plasmid, C-Myc tag||HG10902-CM|
|Human CCNE1 ORF mammalian expression plasmid, C-HA tag||HG10902-CY|
|Human CCNE1 Gene cDNA clone plasmid||HG10902-M|
|Human CCNE1 natural ORF mammalian expression plasmid, HA tag||HG10902-M-Y|
|Human CCNE1 ORF mammalian expression plasmid, N-Flag tag||HG10902-NF|
|Human CCNE1 ORF mammalian expression plasmid, N-His tag||HG10902-NH|
|Human CCNE1 ORF mammalian expression plasmid, N-Myc tag||HG10902-NM|
|Human CCNE1 ORF mammalian expression plasmid, N-HA tag||HG10902-NY|
|Human CCNE1 natural ORF mammalian expression plasmid||HG10902-UT|
|Learn more about expression Vectors|
Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.